Journal: Arthritis and rheumatism

Article Title: Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint.

doi: 10.1002/art.34385

Figure Lengend Snippet: Figure 5. Confocal images showing that CFA-induced inflammation of the joint induces sprouting of NF200, CGRP, and GAP-43 nerve fibers in the synovium and this sprouting is substantially attenuated by anti-NGF therapy. Representative confocal images of NF200- and CGRP-labeled sensory nerve fibers and GAP-43–labeled sprouting nerve fibers (yellow/orange) and DAPI-labeled nuclei (blue) in 20-m–thick knee joint sections from sham (saline)–injected mice (A, D, and G), CFA-injected mice treated with vehicle (B, E, and H), and CFA-injected mice treated with anti-NGF (C, F, and I) are shown. In sham-injected mice, a low-level, regular pattern of innervation by NF200, CGRP, and GAP-43 fibers was observed in the synovial space of the knee joint. Twenty-eight days after the initial injection of CFA, a significant number of NF200, CGRP, and GAP-43 nerve fibers had sprouted and exhibited a disorganized appearance as compared to observations in joints treated with saline only. NF200, CGRP, and GAP-43 sprouted nerve fibers were localized in the synovium and were not observed in the meniscus. Administration of anti-NGF significantly reduced the density of NF200, CGRP, and GAP-43 nerve fibers in the synovium of the joints with CFA-induced arthritis. See Figure 4 for definitions.

Article Snippet: Frozen bone/knee joint sections were incubated with an antibody against calcitonin gene– related peptide (CGRP) (polyclonal rabbit anti-rat CGRP; 1:10,000) (catalog no. C8198; Sigma) to label unmyelinated and thinly myelinated primary afferent sensory nerve fibers, and an antibody against neurofilament 200 kd (NF200) (chicken anti-NF200; 1:5,000) (catalog no. CH22104; Neuromics) to label myelinated primary afferent sensory nerve fibers.

Techniques: Labeling, Saline, Injection

Journal: Arthritis and rheumatism

Article Title: Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint.

doi: 10.1002/art.34385

Figure Lengend Snippet: Figure 4. Anti–nerve growth factor (anti-NGF) reduces Freund’s complete adjuvant (CFA)–induced nerve sprouting of sensory and sympathetic nerve fibers but does not alter the aberrant neovascularization and macrophage infiltration in the arthritic joint. Twenty-eight days after initial injection of CFA, there were significant increases in the density of sensory nerve fibers as demonstrated by positivity for calcitonin gene–related peptide (CGRP) (A) and neurofilament 200 kd (NF200) (B), sympathetic nerve fibers as demonstrated by positivity for tyrosine hydroxylase (TH) (C), and nerve fibers undergoing sprouting as demonstrated by positivity for growth-associated protein 43 (GAP-43) (D), as well as increases in vascularization as demonstrated by CD31 vessels (E) and a significant influx of macrophages (CD68) (F), in the synovium of the arthritic joint. Administration of anti-NGF significantly reduced the density of CGRP and NF200 sensory, TH sympathetic, and GAP-43 sprouting nerve fibers in the synovium of the joints with CFA-induced arthritis. In contrast, preventive sequestration of NGF did not reduce the number or alter the density of the CFA-induced aberrant CD31 neovascularization or the number of CD68 macrophages in the synovium of the arthritic joint. Values are the mean SEM from 6 mice.

Article Snippet: Frozen bone/knee joint sections were incubated with an antibody against calcitonin gene– related peptide (CGRP) (polyclonal rabbit anti-rat CGRP; 1:10,000) (catalog no. C8198; Sigma) to label unmyelinated and thinly myelinated primary afferent sensory nerve fibers, and an antibody against neurofilament 200 kd (NF200) (chicken anti-NF200; 1:5,000) (catalog no. CH22104; Neuromics) to label myelinated primary afferent sensory nerve fibers.

Techniques: Adjuvant, Injection

Journal: Cancers

Article Title: ATX-101, a Peptide Targeting PCNA, Has Antitumor Efficacy Alone or in Combination with Radiotherapy in Murine Models of Human Glioblastoma

doi: 10.3390/cancers14020289

Figure Lengend Snippet: ATX-101 inhibits stemness phenotype and induces a reversion of Neural/proneural to mesenchymal phenotype. ( A ) Confocal analyses of Ki67- and Sox2-stained GSCs-5 cells treated with ATX-101 (1.0 and 2.5 μM) for 48 h. Bar indicates 25 μm. ( B ) FACS analyses for mesenchymal markers CD44 and CD90 in GSCs-5 cells after treatment with ATX-101 (1.0, 2.5, and 5 μM) for 48 h. Percentages of cells positive for CD44, CD90, GAP43, and βIII tubulin after treatment with ATX-101 are summarized in the table below the histograms. ( C ) ICC analyses performed on GSCs-5 cells for CD44, Stro1, NFH, OCT3/4, and GFAP after treatment with ATX-101 (1.0, 2.5, and 5 μM) for 48 h. Bar indicates 10 μm.

Article Snippet: Antibodies against β-actin (sc-69879), PCNA (sc-56), p-Histone H2AX (Ser 139) (γH2A, sc-517348), DNA-PKcs (sc-390849), GFAP (sc-3673), Oct-3/4 (sc-5279), β3 Tubulin (sc-80005), NFH (sc-133237), and Sox2 (sc-365823) were purchased from Santa Cruz (Santa Cruz, CA, USA).

Techniques: Staining

Journal: Journal of Neuroimmune Pharmacology

Article Title: Histamine H3 Receptor Antagonist, Thioperamide, Improves Behavioral and Neuropathological Changes Associated with Subclinical Hypersensitivity to a Cow’s Milk Allergen

doi: 10.1007/s11481-025-10256-9

Figure Lengend Snippet: Fluorescence staining of myelin and neurofilament heavy chain in the anterior cingulate. Brain sections from male ( a ) and female ( b ) sham and BLG mice with vehicle (Veh) and thioperamide (Thio) treatment were stained with FluoroMyelin™ (FM) and neurofilament heavy chain (NF-H) for myelin sheath (red) and axonal structure (green), respectively. DAPI nuclear counterstain was used to identify cell bodies (blue). Representative photomicrographs were taken from the anterior cingulate region of the frontal cortex using a 10× objective. Scale bar = 200 μm. Staining differences among the experimental groups were compared by randomly selecting three samples from each group and quantifying the optical density (OD) of FM staining ( c ) and NF-H immunostaining ( d ) within the anterior cingulate cortex. The bars indicate the relative OD value for each treatment group ± SEM ( n = 3). Sex-disaggregated analyses were performed by 2-way ANOVA, and asterisks between two bars indicate significance (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: The brain sections were subsequently incubated with a rabbit anti-mouse neurofilament heavy chain (NF-H) antibody (Novus Biologicals, Centennial, CO, USA; Catalog # NB300-135, RRID: AB_350460) at a 1:3,000 dilution overnight at 4 °C.

Techniques: Fluorescence, Staining, Immunostaining

Journal: eLife

Article Title: Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones

doi: 10.7554/eLife.37935

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit anti-NF-H , Neuromics , RA22116 , (Immunocytochemistry 1:1,000; Western blot 1:10,000).

Techniques: Immunocytochemistry, Western Blot, Purification, Isolation, Recombinant, Blocking Assay, Expressing, Sequencing, Negative Control, Software